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. Author manuscript; available in PMC: 2009 Sep 10.
Published in final edited form as: Exp Cell Res. 2008 Jun 9;314(15):2884–2894. doi: 10.1016/j.yexcr.2008.06.003

Fig. 1.

Fig. 1

Limd1 protein expression and localization in calvarial osteoblasts.

(A–C) Primary calvarial osteoblasts were cultured in osteogenic conditions for 3, 6, 9, or 12 days, lysed, and equal amounts of protein run on SDS-PAGE then Western blotted for the presence of the Ajuba-LIM family members Limd1 (A), WTIP (B) and Ajuba (C). Actin was used as a loading control. Primary (D) or immortalized (E) Wt or Limd1−/− calvarial osteoblasts were fixed, permeabilized and stained with Limd1 or vinculin antisera. (F) Immortalized wt or Limd1−/−calvarial osteoblasts transduced with RFP or RFP-Limd1, as indicated.