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. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jul 23;374(3):553–558. doi: 10.1016/j.bbrc.2008.07.062

Figure 1. Effects of forskolin on osteoblastic differentiation and calcification.

Figure 1

A) ALP activity and matrix calcium from mouse aortic cells treated for 4 days and 10 days, respectively with or without forskolin (Fsk; 25 µM) in normal or osteogenic medium (normal medium supplemented with 5 mM βGP). Both ALP activity and matrix calcium were normalized to total protein, as described in Methods. B) RT-qPCR analysis of osteopontin (OPN), bone sialoprotein (BSP), osteocalcin (OCN), Cbfa1 and Taz expression from cells treated with control or Fsk (25 µM) for 7 days in osteogenic medium. C) ALP activity and matrix calcification of cells cotreated with control (DMSO) or Fsk (25 µM) and/or H89 (10 µM), as indicated, in the osteogenic medium. D) RT-qPCR analysis of Enpp1, Pit-1 and Ank expression from cells treated with control or Fsk (25 µM) for 7 days in osteogenic medium. *p < 0.05 compared to control; **p < 0.001 compared to control; #p < 0.0001 compared to Fsk-treated.