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. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jul 23;374(3):553–558. doi: 10.1016/j.bbrc.2008.07.062

Figure 2. Effects of phosphate transport on forskolin-induced osteoblastic differentiation and mineralization.

Figure 2

A) Matrix calcification of cells cotreated with control, Fsk (25 µM), PFA (0.5 mM) and/or Lev (10−4 M), as indicated, for 11 days in osteogenic medium. B) Matrix calcification of cells cotreated with control, Pi (3 mM), PFA (0.5 mM), and/or Lev (10−4 M), as indicated, for 11 days in osteogenic medium. C) RT-qPCR analysis of ALP, OPN and OCN expression from cells treated with control or Fsk (25 µM), PFA (0.5 mM), and/or Lev (10−4 M) for 12 days in osteogenic medium. **p < 0.001 compared to non-Fsk-treated control; #p < 0.0001 compared to Fsk-alone-treated.