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. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jul 23;374(3):553–558. doi: 10.1016/j.bbrc.2008.07.062

Figure 3. Effects of pyrophosphate on forskolin-induced osteoblastic differentiation and mineralization.

Figure 3

Matrix calcification of cells treated with control, Pi (2.6 or 3.0 mM), and/or purified porcine ALP (1 unit/mL), as indicated, for 10 days in normal medium. B) Matrix calcification of cells cotreated with control, Fsk (25 µM), PPi (0.1 mM), probenecid (Prob, 1 mM) as indicated in osteogenic medium. C) RT-qPCR analysis of Enpp1 expression from cells treated with control or Fsk (25 µM), or probenecid (1 mM) for 12 days in osteogenic medium. *p < 0.05 compared to control; **p < 0.001 compared to control; #p < 0.0001 compared to Fsk-treated.