TABLE 3.

Reduced Expression of Both p53 and GSTP1 Rendered Resistant Glioblastoma Cells to Cisplatin Treatments

	Control	CDDP
Nonspecific control siRNA	100%	72.5 ± 6.6%
p53 siRNA	100%	45.8 ± 4.5%

NOTE: U87MG cells in 96-well culture plates were transfected with the nonspecific control siRNA or p53 siRNA, and 24 h after transfection, cells were treated with and without 200 μmol/L cisplatin for 24 h and harvested and cell viability was determined using the CellTiter Blue Assay kit. All data represent means and SDs from three independent experiments. Student’s t test was conducted to determine whether a significant difference existed in the survival rate between the nonspecific control siRNA — transfected and p53 siRNA — transfected cells. P value was subsequently determined to be 0.027.