. Author manuscript; available in PMC: 2008 Oct 20.

Published in final edited form as: Mol Immunol. 2007 Jan 10;44(10):2675–2685. doi: 10.1016/j.molimm.2006.12.002

TABLE 1.

Oligonucleotide sequences

	Sequence (5'- 3')	Tm °C
DNA methylation
V1	AGTGTGAGGTGAAGCTGGTGGA ATTATATCTCCAAAATTTCCCTCTAACCAAAA GGTTTATTTTTAGTGATTTTTATATGGAGTGG (nested) ATTATATCTCCAAAATTTCCCTCTAACCAAAA (nested)	55 55
V11	AGTGTGAGGTGAAGCTGGTGGA TACAATTCTATACAATTACCACTATCC TGTGTAATTTTTGGGTTTATTTTTATTGA (nested) TACAATTCTATACAATTACCACTATCC (nested)	55 55
V13	GAGTCAAAATATTGAAGTTTTA AACAATTCTATTCAATTATCAC GTATTATATGAAGTGAGTTTTGA (nested) AACAATTCTATTCAATTATCAC (nested)	49 50
81X^a	GAGAAGAGGCTGGAGTTGGT CTCCATTTTTATCCCTACATTTC	55
Chromatin accessibility
Nla III close to Pax5 site
V107-For V1ac-5' V11ac-5' V13ac-5' V107ac-5' BW4	CACTTTTAMATGGTAATTTATGG GTATGTACCAGCTTTCTCTAC TGAACAAGGGATGCTGAGTATC GGAACAAGGGATGCTGAGTACG GTATCCAGTGTGAGGTGAAG CCGGGAGATCTGAATTCTAG	52 55 55 55 55 55
Nla III close to RSS
V107-5' V1-5' V11-5' V13-5' V107co-5' BW3	TGGGTCCGCCAGCCTCCAG AGACTGGAGTGGATTGCTGCAAG AGGCACTTGAGTGGTTGGGTTTT CAGCCTCCAGGGAAGTCACC AACAGAGTAYAGTGCATC CCGGGAGATCTGAATTCTG	60 55 55 55 47 55
Germline transcription
J558 Vβ5^b Actin	CTAACCATGGGATGGAGCTGGATC TGACGAATTCAGGATGTGGTTACAAC CTCCTGGGAACAAGTTCAGCAA GATTAAGTTACAGAAAGCCAGTAGC TCTGGCTCCTAGCACCATGAAGA GGGACTCATCGTACTCCTGCTTG	60 60 60
Oligos for EMSAs
V1 V11 V13 CD19	GTGCAACTTCTGGGTTCACCTTCAGTGATTTCTACATG CATGTAGAAATCACTGAAGGTGAACCCAGAAGTTGCAC GTGCAACTTCTGGGTTCACCTTCACTGATTACTACATG CATGTAGTAATCAGTGAAGGTGAACCCAGAAGTTGCAC GTGAAGCTTCTGGATTCACCTTCACTGATTACTACATG CATGTAGTAATCAGTGAAGGTGAATCCAGAAGCTTCAC CTAGACAGACACCCATGGTTGAGTGCCCTCCAGT ACTGGAGGGCACTCAACCATGGGTGTCTGTCTAG

Primers were taken from Johnson et al. (2004).

Primers were taken from Fuxa et al. (2004).