TABLE 3.
S. cerevisiae strain | Temp (°C) | Specific metabolite consumption and production rates (mmol C g [dry wt]−1 h−1)
|
Carbon balance | ||||||
---|---|---|---|---|---|---|---|---|---|
Glucoseb,c,d,e | CO2c,d | Succinateb | Glycerolb | Acetateb,c,d | Ethanolb,c,d | Pyruvatec | |||
CEN.PK113-7D | 15 | −24.42 ± 0.25f | 7.92 ± 0.17f | 0.01 ± 0.01 | 0.11 ± 0.01 | 0.17 ± 0.06 | 13.80 ± 0.61 | 0.02 ± 0.01 | 0.95 ± 0.05 |
30 | −31.61 ± 1.00 | 8.71 ± 0.48 | 0.08 ± 0.02 | 0.08 ± 0.00 | 0.08 ± 0.00 | 15.85 ± 0.33 | 0.03 ± 0.01 | 0.86 ± 0.02 | |
EC1118 | 15 | −21.34 ± 0.70 | 7.10 ± 0.40 | 0.03 ± 0.01 | 0.09 ± 0.02 | 0.03 ± 0.01 | 11.98 ± 0.38 | 0.01 ± 0.00 | 0.99 ± 0.02 |
30 | −29.00 ± 0.51 | 8.84 ± 0.22f | 0.05 ± 0.00 | 0.09 ± 0.01 | 0.05 ± 0.02 | 14.56 ± 0.67 | 0.03 ± 0.00 | 0.88 ± 0.01 |
Unless indicated otherwise, the data are the averages ± standard deviations for three independent chemostat steady states.
The means for S. cerevisiae CEN.PK 113-7D cultures grown at 15°C and 30°C are statistically significantly different (P < 0.05) as determined by an unpaired homocedastic t test.
The means for S. cerevisiae EC1118 cultures grown at 15°C and 30°C are statistically significantly different (P < 0.05) as determined by an unpaired homocedastic t test.
The means for S. cerevisiae CEN.PK113-7D and EC1118 cultures grown at 15°C are statistically significantly different (P < 0.05) as determined by an unpaired homocedastic t test.
The means for S. cerevisiae CEN.PK113-7D and EC1118 cultures grown at 30°C are statistically significantly different (P < 0.05) as determined by an unpaired homocedastic t test.
The data are the averages ± standard deviations for two independent chemostat steady states.