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Accumulation of 4-EDF during 2-EHN biodegradation. Parallel cultures were carried out in 120-ml flasks and removed at the times indicated for extraction and measurements of 2-EHN (
) and 4-EDF (▴). CO2 (▪) was measured in a separate culture flask. Residual 2-EHN is the fraction of hydrocarbon that stayed bound to the flask wall and stopper and remained inaccessible to bacteria.
