TABLE 1.
Substrate or product | Mass changeb (mg/liter) | Carbon balance
|
|
---|---|---|---|
Carbon changeb (mg/liter) | Carbon recovery (%) | ||
2-EHN | 482 | 269 | 0 |
Cell biomass | 94 | 50c | 19 |
CO2d | 115 | 31 | 12 |
TOCe | 165 | 165 | 61 |
Total products | 92 |
Cultures (10 ml) were performed at 30°C in 120-ml flasks.
Considering the whole content of the culture flasks.
The carbon/dry-biomass ratio was assumed to be 52% (17). The dry biomass was determined from 100-ml cultures grown in 1-liter flasks.
CO2 was determined after acidification of the culture.
TOC was measured in the culture fluid after filtration through a 0.22-μm membrane.