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. 2008 Sep 5;74(20):6427–6436. doi: 10.1128/AEM.00815-08

TABLE 2.

Fungal oligonucleotide probes and their target organisms, sequences, and target positions

Probe Hybridization stringencya (% formamide) Target organism(s) Probe sequence (5′ to 3′) rRNA subunit, helix,b and binding positionc
MY1574 20 Eumycota TCCTCGTTGAAGAGC 18S; 47; 1474-1489
TCLAD1395 35 Members of the genus Tetracladium CGCACTTCCATTTGCTTG 18S; 44; 1395-1413
TmarchB10 40 Tetracladium marchalianum GCTTAAGGTCAGGGGTAT 28S; B10; 182-200
TmarchC1_1 20 Tetracladium marchalianum TCACCGGATGATCAACTG 28S; C1_1; 605-623
TmarchC1_2 20 Tetracladium marchalianum GCCCATTCCCAGGCCTTT 28S; C1_2; 679-697
ALacumi1698 40 Alatospora acuminata CCGCGCTAGGTGGTCGTT 18S; 49; 1698-1716
TRIang322 35 Tricladium angulatum GCCCACTACGCTACAATC 18S; 12; 322-340
Alongi340 40 Anguillospora longissima CCCGTTGAAACCATAGGT 18S; 12; 340-358
AlongiB16 35 Anguillospora longissima GCGCTGTTCCAAGGGTCT 28S; B16; 345-363
a

Expressed as the percentage of formamide in the hybridization buffer.

b

According to references 8 and 45.

c

Nucleotide position according to Saccharomyces cerevisiae numbering.