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Specific binding of IMPDH VII to the putative mcr and mrt operator regions. Lane 1, free probe; lanes 2 to 4, 0.5, 1, and 2 μg of IMPDH VII, respectively; lane 5, 2 μg of IMPDH VII and 1 μg of poly(dI-dC); lane 6, 2 μg of IMPDH VII and 60 pg of unlabeled probes. Forty-five femtograms of DIG-labeled probes was used for the reactions. All reactions were carried out at 4°C for 2 h.
