TABLE 1.
Synthetic oligonucleotides used in this study as primers or probes
| Name | Sequence (5′-3′) | Experiment(s)a |
|---|---|---|
| mcrCf | ATGATCGGAAAGTGCACT | Northern |
| mcrCr | TCATGCACCTCCTAATGC | Northern |
| mrtDf | ATGATGTCAGAAACAGGA | Northern |
| mrtD52r | CCGATTACCCTTGGATC | Northern |
| 1132-01f | CAGATGCAGGATTCCAGTTA | EMSA |
| 1132-01r | CCGCTTATGCTTCAGTTTTTT | EMSA |
| 1132-02b | GCATAAGCGGTTATATTCAGATTAATACTGGATAATAACGAGATGTAC | EMSA |
| 1132-03f | CGAGATGTACAGTATGGCTT | EMSA |
| 1132-03r | ACTGATGGGGTTATGGGATT | EMSA |
| 1132-04b | CCCCATCAGTTTTATTAATAAAATAGTAAATTTTATTAATAAATAAATAAAACAAGAGGT | EMSA |
| 1132-05b | CGAGATGTACAGTATGGCTTGTTCTATAATGAGCTGTGCTCTGGAG | EMSA |
| 1132-06b | TGCTCTGGAGGTTGGAGGATGAACTTCGGTATAACCTGAAGGTCCATTTCG | EMSA |
| 1132-07b | GTCCATTTCGAGACATTGGTGATGGATAGCTCCTTCATGAGGTGACCATTT | EMSA |
| 1132-08b | GTGACCATTTCCATGGATTATCGCTGGCAATCCCATAACCCCATCAGT | EMSA |
| 1132-09rc | AAATGGTCACCTATGAAGGAG | EMSA |
| 1132-10rc | CGAAATGGACCTTCAGGTTAT | EMSA |
| 1132-11b | TAATGAGCTGTGCTCTGGAGGTTGGAGGATGAACTTCGGT | EMSA |
| 1132-12b | GAACTTCGGTATAACCTGAAGGTCCATTTCGAGACATTGGT | EMSA, affinity |
| 1168-01f | GTCACTCTCTCTGTGGAGA | EMSA |
| 1168-01r | AGTATCATCAGCAGGGAGTA | EMSA |
| 1168-02f | TGATGATACTCTGCTTTGGG | EMSA |
| 1168-02r | TCTGAATCAAGTCGAAAGCTT | EMSA |
| 1168-03b | TTGATTCAGAAAGAAATCTTAATTAATTATAATCAAGTTAAATATGCATCGTATACTAA | EMSA |
| 1168-04f | GTATACTAAGACGGGTGTTTT | EMSA |
| 1168-04r | TTCCGTATACTAAACATCCAG | EMSA |
| 1168-05b | TTGATTCAGAAAGAAATCTTAATTAATTATAATCAAGTTAAATATG | EMSA, affinity |
| 1168-06b | CTTAATTAATTATAATCAAGTTAAATATGCATCGTATACTAAG | EMSA |
| 1168-07b | AGAAAGAAATCTTAATTAATTATAATCAAG | EMSA |
| 1168-08b | AGATTCTAATCTTAATTAATTATAATCAAG | EMSA |
| 1168-11b | AGAAAGAGGTCTTAATTAATTATAATCAAG | EMSA |
| 1168-12b | AGAAAGAAATCTTGGTTAATTATAATCAAG | EMSA |
| 1168-13b | AGAAAGAAATCTTAATTGGTTATAATCAAG | EMSA |
| 1168-14b | AGAAAGAAATCTTAATTAATTATGGTCAAG | EMSA |
| 1168-15b | AGAAAGAAATCTTAATTAATTATAATCGGG | EMSA |
| 126exp10f | ATTCATAATACATATGCATAATGGATCTGCAGGTGd | Expression |
| 126exp10r | GTTAAAAATAGGATCCTCATTCAAGTCCGGCGATGCe | Expression |
a
Northern, Northern blot analysis; affinity, affinity particle purification of DNA-binding proteins; expression, cloning and expression of IMPDH VII.
b
Oligonucleotides were annealed with their complement counterparts to prepare double-stranded EMSA probes.
c
Oligonucleotide used for PCR with forward primer 1132-03f.
d
The underlined sequence is an NdeI site.
e
The underlined sequence is a BamHI site.