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Growth-based agar bulb assay to qualitatively assess motility under high-hydrostatic-pressure and high-viscosity conditions. (A) Swimming motility bulbs under high-pressure conditions (30 MPa) with 0.3% agar. (B) Swarming motility bulbs under high-pressure (30 MPa) and increased-viscosity (0.3% agar with 2.5% PVP-360) conditions. The strains examined were SS9R (the parental strain) and the ΔflaA, ΔflaB, ΔflaC, ΔmotA1, and ΔmotA2 mutants.
