TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| P. profundum | ||
| SS9R | Rifr SS9 derivative | 13 |
| EAE1 | ΔflaA SS9R; Rifr | This study |
| EAE2 | ΔflaB SS9R; Rifr | This study |
| EAE3 | ΔflaC SS9R; Rifr | This study |
| EAE4 | ΔmotA1 SS9R; Rifr (H+ driven) | This study |
| EAE5 | ΔmotA2 SS9R; Rifr (Na+ driven) | This study |
| E. coli | ||
| ED8654 | pRK2073 maintenance | 33 |
| DH5α | RecA−; cloning | 18 |
| XL1-Blue | RecA−; cloning | Stratagene, La Jolla, CA |
| TOP10 | RecA−; cloning | Invitrogen, Carlsbad, CA |
| Plasmids | ||
| pRK2073 | tra genes for conjugal transfer | 9 |
| pRL271 | sacB-containing suicide plasmid; Cmr | 10 |
| pMB2190 | pBR327 derivative; Knr | 2 |
| pEAE1k | flaA deletion construct::pRL271; Knr | This study |
| pEAE2k | flaB deletion construct::pRL271; Knr | This study |
| pEAE3k | flaC deletion construct::pRL271; Knr | This study |
| pEAE4k | motA1 deletion construct::pRL271; Knr | This study |
| pEAE5k | motA2 deletion construct::pRL271; Knr | This study |
a
Knr, kanamycin resistance; Cmr, chloramphenicol resistance; Rifr, rifampin resistance.