TABLE 2.
Oligonucleotide primers used in this study
| Purpose | Primer | Sequencea |
|---|---|---|
| Cloning of α-amylase fragment of apuB in pNZ8048 | ApuAf | TGCATCCCATGGCCCATCACCATCACCATCACCATCACCATCACACCGAGGCCAATGCACAG |
| ApuAr | CTCGAATCTAGATTAGTAGGAGTCCTCGCCGGTG | |
| Cloning of pullulanase-encoding fragment of apuB in pNZ8048 | ApuPf | TGCACGCCATGGCCCATCACCATCACCATCACCATCACCATCACGCCGACACGAGCCGCACCATC |
| ApuPr | TGCGCATCTAGACTACTGTTCGGAGCCCTC | |
| Cloning of internal fragment of apuB | ApuBf | CTTGCGAAGCTTGGCGGCGACGGCTGGGTGAG |
| in pORI19 | ApuBr | GCGACGTCTAGACCTTCGGCGGGCTTGTAGTGC |
| Amplification of 5.1-kb apuB | ApuF | ATGGCCGTACGACGATTCAGCTC |
| fragment for use as probe in Southern hybridization | ApuR | CTGTTCGGAGCCCTCCTTACTG |
| Colony PCR primer pair | Apu1 | ATCGCCATCGCATTCCAAACCAAC |
| pORI19r | GATTAAGTTGGGTAAGCCG |
a
The sequences in bold correspond to restriction sites.