TABLE 1.
Sequence similarities of Clostridium clones corresponding to OTUs detected in raw milk
| OTUa | Most closely related organism(s) | Similarity (%) |
|---|---|---|
| 32 | C. tyrobutyricum | 99 |
| 33 | C. tyrobutyricum | 99 |
| 35 | C. tyrobutyricum | 99 |
| 37 | “C. favososporum,” Clostridium sp. strain 915-1 | 98 |
| 37 | C. tyrobutyricum | 98 |
| 38 | C. sporogenes, C. botulinum | 99 |
| 38 | C. tyrobutyricum | 99 |
| 38 | C. magnum | 98 |
| 39 | “C. favososporum,” Clostridium sp. strain 915-1 | 99 |
| 40 | “C. autoethanogenum,” “C. ragsdalei” | 95 |
| 42 | C. disporicum | 98 |
| 45 | C. disporicum | 99 |
| 46 | C. disporicum | 99 |
| 47 | C. disporicum | 98 |
a
16S rRNA genes from milk samples were amplified by using Clostridium-specific primers (see Materials and Methods) and cloned as 820-bp inserts. Correspondence between clones and OTUs was established by DGGE analysis, and sequences from selected clones were used for comparative analysis against GenBank. Entries for the same OTU appear on separate lines when they correspond to both different clones and different species.