FIG. 6.

Purification of the cyanase from P. pseudoalcaligenes CECT5344 expressed in E. coli DH5α. Cyanase was heterologously expressed in E. coli and purified as indicated in Materials and Methods. The different lanes in the gel subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis correspond to molecular weight markers (lane 1), the cell extract (lane 2), the supernatant after centrifugation of a heated (70°C for 30 min) cell extract (lane 3), and 45% to 60% of the ammonium sulfate fraction of the heated extract (lane 4). The arrow indicates the location of the cyanase on the gel.