TABLE 2.
Cyanase activity in P. pseudoalcaligenes CECT5344 cells grown with different nitrogen sourcesa
| Nitrogen source(s) | Cyanase activity (U/g of protein) |
|---|---|
| Cyanate | 562 ± 53 |
| Ammonium | 0 |
| Ammonium + azideb | 0 |
| Ammonium + cyanate | 0 |
| Nitrate | 0 |
| Nitrate + azideb | 0 |
| Nitrate + cyanate | 495 ± 30 |
| NaCN | 960 ± 64 |
| [Cu(CN)4]2− | 10.000 ± 140 |
| Urea | 1.813 ± 110 |
| l-Arginine | 0 |
| l-Ornithine | 0 |
| −Nc | 41 ± 20 |
| LB | 0 |
| LB + NaCN | 0 |
a
The cells were cultured with the indicated nitrogen sources (10 mM, except cyanide, which was 2 mM) up to the late exponential growth phase. Cyanase activity from the different cell extracts was measured as indicated in Materials and Methods. The data presented are the averages of the results of five independent experiments.
b
Cells were cultured in the presence of 2 mM sodium azide.
c
The minus sign indicates that cells were cultured under nitrogen limitation conditions (2 mM ammonium).