TABLE 1.

PCR primers and control strains used for identification of Amp^r, Tet^r, and Sul^r determinants

Plasmid	Resistance gene	PCR primer sequence (5′ → 3′)	Amplicon size (bp)	Accession no.	Annealing temp (°C)	Reference
SU01	pse1	CGC TTC CCG TTA ACA AGT AC CTG GTT CAT TTC AGA TAG CG	419	M69058	60	18
SU05	oxa1	AGC AGC GCC AGT GCA TCA ATT CGA CCC CAA GTT TCC	708	AJ009819	60	18
SU07	tem1	TTG GGT GCA CGA GTG GGT TAA TTG TTG CCG GGA AGC	503	AF126482.1	60	18
pSL18	tet(A)	GCT ACA TCC TGC TTG CCT TC CAT AGA TCG CCG TGA AGA GG	210	X61367	59.5	31
pRT11	tet(B)	TTG GTT AGG GGC AAG TTT TG GTA ATG GGC CAA TAA CAC CG	659	J01830	59.5	31
pBR322	tet(C)	CTT GAG AGC CTT CAA CCC AG ATG GTC GTC ATC TAC CTG CC	418	J01749	59.5	31
pSL106	tet(D)	AAA CCA TTA CGG CAT TCT GC GAC CGG ATA CAC CAT CCA TC	787	L06798	59.5	31
pSL1504	tet(E)	AAA CCA CAT CCT CCA TAC GC AAA TAG GCC ACA ACC GTC AG	278	L06940	59.5	31
pJA8122	tet(G)	GCT CGG TGG TAT CTC TGC TC AGC AAC AGA ATC GGG AAC AC	468	S52437	59.5	31
PAT102	tet(K)	TCG ATA GGA ACA GCA GTA CAG CAG ATC CTA CTC CTT	169	S67449	59.5	31
pVB.A15	tet(L)	TCG TTA GCG TGC TGT CAT TC GTA TCC CAC CAA TGT AGC CG	267	U17153	59.5	31
pJ13	tet(M)	GTG GAC AAA GGT ACA ACG AG CGG TAA AGT TCG TCA CAC AC	406	X90939	59.5	31
pUOA1	tet(O)	AAC TTA GGC ATT CTG GCT CAC TCC CAC TGT TCC ATA TCG TCA	515	Y07780	59.5	31
pAT451	tet(S)	CAT AGA CAA GCC GTT GAC C ATG TTT TTG GAA CGC CAG AG	667	C92946	59.5	31
pJIR39	tetA(P)	CTT GGA TTG CGG AAG AAG AG ATA TGC CCA TTT AAC CAC GC	676	L20800	59.5	31
pNFD13-2	tet(Q)	TTA TAC TTC CTC CGG CAT CG ATC GGT TCG AGA ATG TCC AC	904	X58717	59.5	31
pBS5	tet(X)	CAA TAA TTG GTG GTG GAC CC TTC TTA CCT TGG ACA TCC CG	468	M37699	59.5	31
amr130	sul1	CGG CGT GGG CTA CCT GAA CG GCC GAT CGC GTG AAG TTC CG	433	AF071413	69	25
amr130	sul2	CGG CTC AAG GCA GAT GGC ATT GCG TTT GAT ACC GCG ACC CGT	285	M36657	69	25
rlo44	sul3	GAG CAA GAT TTT TGG AAT CG CTA ACC TAG GGC TTT GGA TAT	790	AJ459418	53	19