TABLE 5.
Gene expression analysis in proximal colon mucosa of pigs treated with Bb12
| TLR | Slope for treatment groupa:
|
|||
|---|---|---|---|---|
| C/C | C/T | T/C | T/T | |
| 2 | −0.03 | 0.12 | 0.09 | −0.28 |
| 4 | −0.03 | 0.04 | −0.006 | −0.16 |
| 9 | 0.26 | 0.12 | −0.04 | −0.89b |
a
Slopes of linear regression lines constructed with CT values obtained for mRNA expression at different times (days 7, 19, 32, and 91). Changes in gene expression over time are determined by comparing differences in the slope of the regression lines that represented each treatment. A negative slope will indicate an upregulation of the gene since the CT value is inversely correlated to the amount of gene expressed in the original sample.
b
Statistical significance is noted when P was <0.05.