Fig. 3.

Effects of cardiac arrest and resuscitation on canine frontal cortex PDHC enzyme activity. PDHC activity remains unchanged after 10 min of cardiac arrest (CA) alone. Activity is decreased significantly, however, as early as 30 min of reperfusion (Re) and remains depressed through 24 hr. PDHC activity was measured using a radioisotopic assay that monitors CO₂ production from [1-¹⁴C] pyruvate. PDHC activity is reported in nmol/min/mg total brain protein. Reprinted from Free Radical Biology and Medicine, Vol 16, Bogaert et al., Postischemic inhibition of cerebral cortex pyruvate dehydrogenase, p 811–820, ^©1994 with permission from Elsevier.