Figure 2. Transcription regulation of MCP-1 by p27^SJ.

A, B, C. Human microglial (A and C) and U-87MG (B) cells were transfected with 0.5 µg of MCP-1-CAT reporter plasmid alone or combined with increasing amounts of transfected (A, B) or infected with adeno-p27^SJ (C) as indicated. Adeno-null was used as a negative control. CAT activity was determined 24 h after transfection. D, E. Twenty micrograms of extracts prepared from non-transfected, p27^SJ-transfected U-87MG cells (D), or from uninfected or p27^SJ-infected cells (E) were subjected to Western blot analysis using anti myc antibody. The arrows point to the position of p27^SJ. The values shown on the top of each bar represent the fold activation over the basal promoter activity, which is arbitrarily set at one. The data represent the mean value of at least three separate transfection experiments.