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. Author manuscript; available in PMC: 2009 Aug 29.
Published in final edited form as: J Mol Biol. 2008 May 29;381(2):351–360. doi: 10.1016/j.jmb.2008.05.056

Figure 4.

Figure 4

g(s*) analysis of PKR binding to dsRNA. A) PKR binding to a 30 bp dsRNA in 200 mM NaCl. Normalized g(s*) distributions of 0.75 µM dsRNA (black), dsRNA + 1 eq. PKR (blue), dsRNA + 2 eq. PKR (red), dsRNA + 3 eq. PKR (green), dsRNA + 6 eq. PKR (tan). B) PKR binding to a 20 bp dsRNA in 75 mM NaCl. Normalized g(s*) distributions of 1 µM dsRNA (black), dsRNA + 0.5 eq. PKR (orange), dsRNA + 1 eq. PKR (blue), dsRNA + 2 eq. PKR (red), dsRNA + 4 eq. PKR (grey). Conditions: rotor speed, 40,000 RPM; temperature, 20°C; wavelength, 260 nm; scan interval, 5 minutes.