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. 2008 Nov;57(11):3090–3098. doi: 10.2337/db08-0564

FIG. 3.

FIG. 3.

A: Representative Western blot depicting the effect of high glucose on MyD88 and IRAK-1 phosphorylation in THP-1 cells. After culturing cells with high glucose, cells were lysed, and cell lysates were blotted for MyD88, total and phospho–IRAK-1, and β-actin as described in research design and methods (n = 4). B: Representative Western blot showing enhanced expression of MyD88, IRAK-1, and pIRAK-1 in THP-1 cell lysates immunoprecipitated with TLR2 antibody after high-glucose challenge as detailed in research design and methods. β-Actin was used as a loading control, n = 4 experiments. C: Representative Western blot showing enhanced expression of MyD88, IRAK-1, and pIRAK-1 in THP-1 cell lysates immunoprecipitated with TLR4 antibody after high-glucose challenge as detailed in research design and methods. β-Actin was used as a loading control, n = 4 experiments.