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. 2008 Nov;57(11):3090–3098. doi: 10.2337/db08-0564

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Copyright © 2008, American Diabetes Association

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 5. — A: IL-1β concentration in supernatants of THP-1 cells after high-glucose treatment in the absence of TLR2 and TLR4 was measured using ELISA. Values are normalized to milligrams cell protein. *P < 0.05 vs. mannitol; **P < 0.05 vs. Sc + high glucose (HG); Sc, scramble control siRNA; TLR2si, TLR2 receptor siRNA; TLR4si, TLR4 receptor siRNA. n = 4 experiments in duplicate. B: IL-6 concentration in supernatants of THP-1 cells after high-glucose treatment in the absence of TLR2 and TLR4 was measured using ELISA. Values are normalized to milligrams cell protein. *P < 0.05 vs. mannitol; **P < 0.05 vs. Sc + HG; Sc, scramble control siRNA; TLR2si, TLR2 receptor siRNA; TLR4si, TLR4 receptor siRNA. n = 4 experiments in duplicate. C: MCP-1 concentration in supernatants of THP-1 cells after high-glucose treatment in the absence of TLR2 and TLR4 was measured using ELISA. Values are normalized to milligrams cell protein. *P < 0.05 vs. mannitol; **P < 0.05 vs. Sc + HG; Sc, scramble control siRNA; TLR2si, TLR2 receptor siRNA; TLR4si, TLR4 receptor siRNA. n = 4 experiments in duplicate. D: TNF-α IL-1β concentration in supernatants of THP-1 cells after high-glucose treatment in the absence of TLR2 and TLR4 was measured using ELISA. Values are normalized to milligrams cell protein. *P < 0.05 vs. mannitol; **P < 0.05 vs. Sc + HG; Sc, scramble control siRNA; TLR2si, TLR2 receptor siRNA; TLR4si, TLR4 receptor siRNA. n = 4 experiments in duplicate. □, Mannitol; , LG (5.5 mmol/l); ▪, HG (15 mmol/l).

Inline graphic — A: IL-1β concentration in supernatants of THP-1 cells after high-glucose treatment in the absence of TLR2 and TLR4 was measured using ELISA. Values are normalized to milligrams cell protein. *P < 0.05 vs. mannitol; **P < 0.05 vs. Sc + high glucose (HG); Sc, scramble control siRNA; TLR2si, TLR2 receptor siRNA; TLR4si, TLR4 receptor siRNA. n = 4 experiments in duplicate. B: IL-6 concentration in supernatants of THP-1 cells after high-glucose treatment in the absence of TLR2 and TLR4 was measured using ELISA. Values are normalized to milligrams cell protein. *P < 0.05 vs. mannitol; **P < 0.05 vs. Sc + HG; Sc, scramble control siRNA; TLR2si, TLR2 receptor siRNA; TLR4si, TLR4 receptor siRNA. n = 4 experiments in duplicate. C: MCP-1 concentration in supernatants of THP-1 cells after high-glucose treatment in the absence of TLR2 and TLR4 was measured using ELISA. Values are normalized to milligrams cell protein. *P < 0.05 vs. mannitol; **P < 0.05 vs. Sc + HG; Sc, scramble control siRNA; TLR2si, TLR2 receptor siRNA; TLR4si, TLR4 receptor siRNA. n = 4 experiments in duplicate. D: TNF-α IL-1β concentration in supernatants of THP-1 cells after high-glucose treatment in the absence of TLR2 and TLR4 was measured using ELISA. Values are normalized to milligrams cell protein. *P < 0.05 vs. mannitol; **P < 0.05 vs. Sc + HG; Sc, scramble control siRNA; TLR2si, TLR2 receptor siRNA; TLR4si, TLR4 receptor siRNA. n = 4 experiments in duplicate. □, Mannitol; , LG (5.5 mmol/l); ▪, HG (15 mmol/l).