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. 2008 Oct 21;6(10):e253. doi: 10.1371/journal.pbio.0060253

Figure 2. MEK and GSK3 Inhibitors (2i) Promote Reprogramming to Full Pluripotency.

Figure 2

(A) Example of 2i-iPS cell colonies generated from aNS cells replated at day 5 after infection in 2i medium.

(B) Plates containing control and infected aNS cells and MEFs were cultured in 2i media from day 3 or day 5 after infection and stained for AP 10 d later. Numbers of AP-stained colonies are indicated.

(C) Established 2i-iPS cell line.

(D and E) Conventional (D) and quantitative (E) RT-PCR analyses of 2i-iPS cells generated from aNS and fNS Oct4GFP NS cells, and from fNS cells of non-transgenic C57BL/6 strain background.

(F) Immunofluorescence staining for Oct4 and Nanog in 2i-iPS cell colony after first passage.

(G) Immunostaining forme3K27. A cluster of residual Oct4-negative cells retains the nuclear body of an inactive X chromosome, providing an internal control.

(H) Chimera generated from injection of 2i-iPS (aNS) cells into C57BL/6 host blastocyst.