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. 2008 Oct 30;3(10):e3574. doi: 10.1371/journal.pone.0003574

Figure 1. Schematic representation of the database mining for miRNA primary transcripts.

Figure 1

MiRNA precursor sequences were used as a query to search the mouse genome database. After locating the miRNA precursor in the genome, a 3 kb sequence centered miRNA precursor was extracted and used as a query to search EST and non-coding RNA databases. After filtering the Blast hits, the EST surrounding the miR-precursor was used as template to design RACE primers to identify the transcriptional initiation site (TIS) and transcriptional termination site (TTS).