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The mutants were constructed by replacing the H. neapolitanus genes for large (cbbL) and small (cbbS) subunit (green boxes) with noncarboxysomal (Tc NC; red boxes) or carboxysomal (Tc C; orange boxes) genes from T. crunogena or replacing both genes with a kanamycin resistance cassette (kanr; white boxes). All mutants carry a kanamycin cassette for selection purposes.
