Figure 11.

Release of galectin-4 from mucosal explants. Mucosal explants of about 0.1 g (wet weight) were excised and placed in culture dishes and immersed in 1 ml of ice-cold Hanks’ buffered salt solution (HBSS). After 15 min, the HBSS was collected and replaced by 1 ml of ice-cold HBSS containing 100 mM sucrose (+Suc). After 15 min, this solution was replaced by 1 ml of ice-cold HBSS containing 100 mM lactose (+Lac) and incubated 15 min. Protein released by the three wash solutions was precipitated by addition of an equal volume of acetone and pelleted by centrifugation at 20,000 × g for 10 min, and equal amounts of sample from each wash were analyzed by SDS-PAGE with a sample of the mucosal explant (Exp). After electrophoresis and transfer to Immobilon, galectin-4 and aminopeptidase N were visualized by Western blotting. Molecular mass values are indicated.