Figure 2.

Brush border enzymes in high molecular weight clusters. Rocket immunoelectrophoresis against antibodies to aminopeptidase N, sucrase-isomaltase, maltase-glucoamylase, aminopeptidase A, and lactase-phlorizin hydrolase of gradient fractions from top to bottom of the experiment shown in Figure 1. Twenty microliters of each fraction was applied to the wells and after electrophoresis, the immunoprecipitates were visualized by staining with Coomassie brilliant blue.