Figure 3.

Lactose releases galectin-4 from high molecular weight clusters. An experiment similar to that shown in Figure 1 except that the membranes were detergent extracted in the presence of 100 mM lactose and that lactose (10 mM) was present in the sucrose gradient. After SDS-PAGE and electrotransfer onto Immobilon, the bands of galectin-4 and aminopeptidase N were visualized by Western blotting. Molecular mass values are indicated.