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. 1997 Nov;8(11):2241–2251. doi: 10.1091/mbc.8.11.2241

Figure 4.

Figure 4

Galectin-4 is a major component of glycolipid microdomains. SDS-PAGE of Triton X-100-insoluble complexes prepared from a microvillar fraction. After electrophoresis and electrotransfer onto an Immobilon membrane, the gel tracks were either stained for protein with Coomassie brilliant blue (lane 1) or Western blotted, using a monoclonal antibody to annexin II (lane 2) or a polyclonal antibody to galectin-4 prepared as described in MATERIALS AND METHODS (lane 3). Notice that the galectin-4 antibody only reacted with the 36-kDa band and not the 265- and 166-kDa bands of sucrase-isomaltase and aminopeptidase N, respectively. Molecular mass values are indicated.