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. 2008 Sep 30;99(8):1322–1329. doi: 10.1038/sj.bjc.6604688

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Copyright © 2008 Cancer Research UK

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Lysophosphatidic acid (LPA)-induced NET1 expression drives AGS cell invasion by RhoA activation. (A) Effect of LPA, NET1 siRNA and C3 exoenzyme on NET1 mRNA expression. Cells were treated with siRNA for 48 h before treatment with or without LPA or C3 exoenzyme, 4 h after which cells were lysed and analysed by real-time PCR. Error bars represent the standard deviation of triplicate experiments. (B) Western blot analysis showing the effect of LPA, NET1 siRNA and C3 exoenzyme on levels of ‘active’ and total RhoA and β-actin. Cells were treated with siRNA for 48 h before treatment with or without LPA or C3, 4 h after which cells were lysed and analysed bya western immunoblot. All immunoblot analysis was repeated in triplicate. (C) The in vitro migration of AGS cells treated with LPA, NET1 siRNA and C3 exoenzyme. Cells were treated with siRNA for 48 h before treatment with or without LPA or C3, 24 h after which the effect on migration was assessed. Error bars represent the standard deviation of triplicate experiments. (D) The effect of LPA, NET1 siRNA and C3 exoenzyme on the in vitro invasion of AGS cells. Cells were treated with siRNA for 48 h before treatment with or without LPA or C3, 24 h after which the effect on invasion was assessed. Error bars represent the standard deviation of triplicate analysis (^*P<0.05).