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. 2008 Oct 14;99(8):1296–1301. doi: 10.1038/sj.bjc.6604680

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Copyright © 2008 Cancer Research UK

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(A) shows an example of fluorescent in situ hybridisation for chromosome 10 (red signal) and PTEN (green signal). (B) Shows an example of immunohistochemistry for PTEN protein expression; in hormone-sensitive prostate cancer, both cytoplasmic expression and nuclear expression are present. (C) Western blot analysis for PTEN protein to confirm antibody specificity. LNCaP cells do not express PTEN protein, whereas DU145 cells do express PTEN protein. Lane 1, hormone-sensitive LNCaP cell lysates; lane 2, hormone-refractory LNCaP cell lysates; lane 3, DU145 cell lysates.