Table 1.
Relative Activity of PMT Mutants
| Mutant | Mitogenicity | Inositol trisphosphate induction | Stress fibre formation |
|---|---|---|---|
| H1202L | 57 | 56 | + |
| H1205L | 1 | 1 | - |
| H1223L | 10 | 3 | - |
| H1228L | 45 | 18 | + |
| H1202Y | 96 | 98 | ++ |
| H1205Y | 1 | 8 | - |
| H1223Y | 52 | 23 | +++ |
| H1228Y | 83 | 80 | +++ |
Summary of data in Figs. 1 and 2. The values given for mitogenicity are the average of induced thymidine incorporation relative to that induced by 10% foetal calf serum at 1, 10 and 100ngml-1 PMT (Fig. 1). Inositol trisphosphate induction is the induction above the untreated value relative to that induced by 10% foetal calf serum (Fig. 2). The induction of stress fibres formation is scored on the basis of-no induction;+evidence of stress fibre formation; ++ between+and +++; +++ stress fibre induction equivalent to that induced by PMT (Fig. 2)