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Heparin affinity and uPA inhibition of native and heated Serp-1 Proteins
| Samples | Proportion*(%) | Heparin Affinity**(mM NaCl) | uPA Inhibition |
|---|---|---|---|
| Native Serp-1 | |||
| Unbound | 5 | 0 | No |
| Peak 1 | 95 | 555 | Yes |
| Heated Serp-1 | |||
| Unbound | 6 | 0 | No |
| Peak 1 | 86 | 400 | No |
| Peak 2 | 8 | 680 | Yes |
*Protein concentration was measured using the Bio-Rad Protein Assay Kit
**Heparin affinity is given as the salt concentration required for elution
***Measured in uPA activity assay
