Fig. (2).

Chromatogram showing the UV absorption profile (λ = 254 nm) of Arabidopsis supernatant separated on Sephadex G-50 Superfine. Gel volume: 500 mL; column length: 700 mm; column diameter: 30 mm; eluent flow rate: 12 mL / hr; fraction volume: 8.0 mL; number of fractions: 95; sample volume: 5 mL; separation temperature: 4 °C; elution buffer: 20 mM Tris-HCl, 1 mM NaN3; pH 8.0. The peripheral tools used for preparative native GPC are listed in [23, 35]. The denoted molecular weights of the detected metal compounds (MW ≥ 30 kDa) are approximated values. Metal cofactors eluted in the range of the void volume (120 to 140 mL) of this method were identified and quanti-fied by ICP-MS or GF-AAS [23, 35].