Table 1.

Michaelis Constants of Different Phagemid Prepara-tions and of Soluble PhoA

Sample	c(IPTG) [µM]	Km [µM]d)
pIII-PhoA	0	11.2 (± 0.1)
pIII-PhoA	1	12.3 (± 0.9 )
pIII-PhoA	10	9.0 (± 0.8 )
pIII-PhoA	100	9.8 (± 1.1 )
pIII-PhoA	1000	10.1 (± 1.2 )
pVIII-PhoA	0	12.9 (± 1.0 )
pVIII-PhoA	1	12.9 (± 1.2 )
pVIII-PhoA	10	12.3 (± 0.9)
pVIII-PhoA	100	11.0 (± 0.7)
pVIII-PhoA	1000	11.0 (± 0.6)
PhoA a)		12.3 (± 0.2)
PhoAb)		12.7
PhoAc)		8.5

^a)Km from the present work, ^b)Km from [ 24], ^c)Km from [ 23]. ^d)The standard deviation derived from three experiments performed with independent freshly prepared phagemids is given in parentheses