. Author manuscript; available in PMC: 2008 Dec 4.

Published in final edited form as: Biochemistry. 2007 Nov 8;46(48):13819–13828. doi: 10.1021/bi701249q

Table 1.

Results from the coupled enzyme assay using (R)-2 as the substrate for GABA-AT. Previous data regarding the kinetic constants for GABA and racemic 3-F-GABA (1) are also shown.

Substrate	K_m (mM)	V_max (µmol/min*mg)	V_max/K_m (L/min*mg)

GABA	2.6^a [2.1]^b	8.9 [12.1]	0.0034 [0.0058]
(±) 3-F-GABA-1^c	0.045 [0.063]	1.3 [1.4]	0.0289 [0.0222]
(R)-2	0.059 [0.050]	4.93 [5.28]	0.0836 [0.1056]
(S)-2	Could not be determined^d	Could not be determined^d	Could not be determined^d

Kinetic constants not in brackets were determined using a Hanes-Woolf plot.

Kinetic constants shown in brackets were determined using a Lineweaver-Burk plot.

See reference 31.

The rate was too slow for an accurate measurement.