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Inhibition of GABA transamination by the enantiomers of 3-F-GABA (2) using the coupled enzyme assay. Succinic semialdehyde production via turnover of 3-F-GABA was accounted for by subtracting the rate of succinic semialdehyde production for each 3-F-GABA enantiomer from the observed rate of GABA transamination in the presence of that enantiomer.
| Reaction | Rate (change in abs/min) | % activity remaining |
|---|---|---|
| GABA (1) | 0.142 | 100 |
| GABA + 1 mM (R)-2 | 0.012 | 8 |
| GABA + 1 mM (S)-2 | 0.122 | 86 |
