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Short-term effects of RMT 5265 treatment in C666-1 NPC cells. Flow cytometry assessment of DNA content and counting of cells with sub-G1 characteristics. Treatment with 50 nM RMT 5265 for 48 hours increased the fraction of sub-G1 cells to 30% with no substantial change at a higher concentration of RMT 5265 (200 nM). In contrast, the fraction of sub-G1 cells was not increased by RMT 52656 in non-NPC carcinoma cells (HeLa) or in nonmalignant nasopharyngeal epithelial cells (NP69).
