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. 2008 Nov;10(11):1183–1194. doi: 10.1593/neo.08590

Figure 7.

Figure 7

Caspase-8 activation induced by TLR3 stimulation combined with IAP RNA interference. (A) Impact of siRNA on the cellular concentration of c-IAP2, XIAP, and survivin assessed by Western blot detection (specific protein bands were quantified by film densitometry using a GS-710 calibrated imaging densitometer with the Quantity One software; Biorad). (-) indicates no siRNA; NS: non-specific siRNA. (B) Caspase-8 assay (chemiluminescence), c-IAP2, XIAP, and FLIP-L detection (Western blot) and PARP cleavage assay (Western blot) in C666-1 undergoing knockdown of various IAPs with or without poly(I:C) treatment. Cells underwent two rounds of siRNA transfection at 48-hour intervals. NS indicates nonspecific siRNA. Treatment with poly(I:C) was started 32 hours after the second transfection, and cells were collected 16 hours later for protein extraction and caspase-8 assay. Each measurement was done in duplicate. The star indicates a statistical difference from nonspecific siRNA combined with poly(I:C) (P < .05).