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. 2008 Nov;10(11):1195–1203. doi: 10.1593/neo.08640

Figure 4.

Figure 4

Methylation of the proximal promoter of the PLA2G2A gene. (A) Location of 5′-CpG sites and potential binding regions for transcription factors in the proximal promoter of PLA2G2A gene was assessed using MethPrimer and TFSEARCH software as described in the Materials and Methods section. The gene sequence is numbered [-900, +200 bp] relative to the transcription start site [29] and the proximal promoter region [-260, +20] of PLA2G2A. Positions of potential binding sites for transcription factors, STAT, NF-κB, C/EBP-β, NF-Y, Sp1, and γ-IRE, are illustrated. (B) Methylation status of selected 5′-CpG sites in the proximal PLA2G2A promoter of Jurkat genomic DNA before and after exposure to 10 µM 5-aza-dC as described in the Materials and Methods section. Representative results of sequence analyses of the bisulfite-treated genomic reverse DNA strands are shown. Arrows indicate the conversion of reverse 5′-CpG sites, -186, -111, -82, and +8, in dependence on the methylated status. (C) Methylation-specific PCR analyses of genomic DNA before and after exposure of Jurkat cells to 5-aza-dC. M or U indicate the presence of methylated or unmethylated 5′-CpG sites.