Figure 7.

Down-regulation of YAP decreased proliferation in merlin-deficient cells. MENII-1-Control and MENII-1-NF2-siRNA cells were transiently transfected with YAP-specific siRNA or empty vector. (A) YAP transcript levels were measured in MENII-1-Control cells using quantitative PCR. The YAP-specific siRNA caused a 50% reduction in YAP transcript levels compared with controls in MENII-1 cells. (B, C) BrdU incorporation and 7-AAD staining were measured by flow cytometry. (B) One representative experiment shows the distribution of cells in G₀-G₁, S, and G₂ phases of the cell cycle. The table below shows the mean of the percentage of cells ±SE in each phase of the cell cycle fromthree independent experiments. (C) Bar graphs depict the percentage of cells in the S-phase of the cell cycle (BrdU-positive cells) averaged from three independent experiments. Error bars correspond to ±SE. Suppression of YAP decreased the percentage of cells in S-phase in MENII-1-NF2-siRNA cells to levels similar to MENII-1-Control cells.