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Decreased expression of the NF proteins in TEC3KO schwannomas. Frozen tissue sections from the same tumor were stained by immunofluorescence for Prkar1a, neurofibromin, and merlin proteins. Tumor stroma (S) stained positively and was therefore used as a control for staining. The dashed line marks the interface between stromal and tumor tissues. As expected, Prkar1a was not present in the tumor tissue (T), but surprisingly both neurofibromin and merlin were absent as well.
