Figure 12.

Anti-CD81 antibody blocks migration on Ln-1 and Fn. Transwell filters were coated for 4 h at 37°C with 20 μg/ml Ln-1 or 20 μg/ml Fn. ES6A, ES6B, or ESneo cells (1 × 10⁴ cells/filter) were plated on the uncoated side. Cells were incubated with 75 μg/ml hamster anti-CD81 (2F7) or, for controls, cells were incubated with 2% glycerol in PBS (since 2F7 is supplied in 20% glycerol) or with 75 μg/ml hamster anti-TNP antibody in migration medium for 30 min. No antibodies were removed before plating on the filters. In all cases, cells were maintained at 37°C for 18 h, and then filters were fixed, stained, and analyzed as previously described.