Figure 5.

ES6A cell migration on Ln-1, Fn, and Ln-5 is blocked by anti-α6 mAb GoH3. Transwell filters were coated for 4 h at 37°C with 20 μg/ml Ln-1, 20 μg/ml Fn, or 10 μg/ml Ln-5. ES6A, ES6B, or ESneo cells (1 × 10⁴ cells/filter) were plated on the uncoated side. For blocking experiments, cells were incubated at room temperature with 10 μg/ml GoH3 antibody in migration medium for 30 min before plating on coated filters. Cells were maintained at 37°C for 18 h, and then filters were fixed and stained. To quantify migration, stained cells were counted in four fields (by using a 40× objective) from each of two filters for each condition. Results of representative experiments are expressed as the number of cells counted in each field (mean ± SD).