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Monensin does not abolish α6A-induced migration on Fn. Transwell filters were coated for 4 h at 37°C with 40 μg/ml Ln-1, 40 μg/ml Fn, or 10 μg/ml Ln-5. ES6A, ES6B, or ESneo cells (1 × 104 cells/filter) were plated on the uncoated side of filters in migration medium, or migration medium including 0.01, 0.1, or 1 μM monensin. In all cases, cells were maintained at 37°C for 18 h, and then filters were fixed, stained, and analyzed as previously described.
