Figure 5.

Synergistic cytotoxic effect of MJ and 2-DG toward MCA-105 cells. MCA-105 cells were seeded into 96-well plates at a concentration of 3 x 10³ cells per well and were allowed to adhere overnight. After incubation, vehicle (ethanol), 2-DG (1, 2 mM), and MJ (0.5, 1 mM, added 1 hour later) were added to the wells for 16 hours. The cytotoxic effect was measured using the XTT Cell Proliferation Kit as described in the Materials and Methods section. Values are means ± SD of at least three experiments. * Indicates that the experimentally measured combined cytotoxic effect is greater than the expected additive effect as calculated using the Bliss additivism model.