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. 2008 Nov;10(6):520–526. doi: 10.2353/jmoldx.2008.080024

Figure 2.

Figure 2

PNA-clamp SMAP-2 primer set sequences for the KRAS mutation codon 12 detection assay. A: Sequence alignment of the KRAS mutation at codon 12 and SMAP-2 primer positioning. Wild-type sequence of KRAS gene is illustrated. The arrowheads indicate the position of the mutations found in codon 12. DNA sequences used for primer design are marked. B: SMAP-2 primer sequences for the KRAS mutation at codon 12 detection assay. Unlike other SMAP-2 assays previously reported, none of the sequences are a discrimination probe, since they bracket the actual site of sequence variability.